lasergene genomics suite version 15 Search Results


powassan viral genomic sequences  (DNASTAR)
99
DNASTAR powassan viral genomic sequences
Genomic sequence analysis and infectious U118 neuronal cell culture system (A) Phylogenetic analysis of five <t>Powassan</t> viral genome sequences from known lineage I and II strains. GenBank accession numbers are provided along with strain information including location and year of isolation. Phylogenetic tree was constructed using the neighbor-joining method with pairwise distance estimation following ClustalW alignment program. (B) Matrix panel shows the percent identity in the upper right <t>and</t> <t>nucleotide</t> divergence in the lower left of aligned Powassan viral genomic sequences (ClustalW program, DNASTAR Lasergene MegAlign 15). (C) Graph shows the RT-qPCR analysis of POWV (LB), POWV ( M11665 ), and DTV (SPO) genome replication in U118 cells 24 and 48 hpi. The y axis shows genome copies per 50 ng of total RNA in log scale. Error bars represent the SD of three biological replicates. Student’s t test. ∗ p < 0.01, ∗∗ p < 0.001, ∗∗∗ p < 0.0001, ∗∗∗∗ p < 0.00001. (D) Representative immunofluorescence images of mock and LB-, DTV- ( M11665 ), and DTV- (SPO) infected U118 neuronal cells detected by viral NS1 at 24 and 48 hpi (scale bars, 25 μm). Red, NS1 viral protein; blue, DAPI. (E) Western blot analysis of infected cell lysates probed with the anti-M protein antibody targeting LB, DTV ( M11665 ), and DTV (SPO) at 24 and 48 hpi. Cells were infected with each virus at an MOI of 1. Two independent experiments were conducted. Representative data are presented.
Powassan Viral Genomic Sequences, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lasergene+genomics+suite+version+15/pmc12677074-122-36-42?v=DNASTAR
Average 99 stars, based on 1 article reviews
powassan viral genomic sequences - by Bioz Stars, 2026-08
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dnastar lasergene  (DNASTAR)
97
DNASTAR dnastar lasergene
Genomic sequence analysis and infectious U118 neuronal cell culture system (A) Phylogenetic analysis of five <t>Powassan</t> viral genome sequences from known lineage I and II strains. GenBank accession numbers are provided along with strain information including location and year of isolation. Phylogenetic tree was constructed using the neighbor-joining method with pairwise distance estimation following ClustalW alignment program. (B) Matrix panel shows the percent identity in the upper right <t>and</t> <t>nucleotide</t> divergence in the lower left of aligned Powassan viral genomic sequences (ClustalW program, DNASTAR Lasergene MegAlign 15). (C) Graph shows the RT-qPCR analysis of POWV (LB), POWV ( M11665 ), and DTV (SPO) genome replication in U118 cells 24 and 48 hpi. The y axis shows genome copies per 50 ng of total RNA in log scale. Error bars represent the SD of three biological replicates. Student’s t test. ∗ p < 0.01, ∗∗ p < 0.001, ∗∗∗ p < 0.0001, ∗∗∗∗ p < 0.00001. (D) Representative immunofluorescence images of mock and LB-, DTV- ( M11665 ), and DTV- (SPO) infected U118 neuronal cells detected by viral NS1 at 24 and 48 hpi (scale bars, 25 μm). Red, NS1 viral protein; blue, DAPI. (E) Western blot analysis of infected cell lysates probed with the anti-M protein antibody targeting LB, DTV ( M11665 ), and DTV (SPO) at 24 and 48 hpi. Cells were infected with each virus at an MOI of 1. Two independent experiments were conducted. Representative data are presented.
Dnastar Lasergene, supplied by DNASTAR, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lasergene+genomics+suite+version+15/pmc12288884-593-9-9?v=DNASTAR
Average 97 stars, based on 1 article reviews
dnastar lasergene - by Bioz Stars, 2026-08
97/100 stars
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lasergene genomics suite version 15  (DNASTAR)
94
DNASTAR lasergene genomics suite version 15
Genomic sequence analysis and infectious U118 neuronal cell culture system (A) Phylogenetic analysis of five <t>Powassan</t> viral genome sequences from known lineage I and II strains. GenBank accession numbers are provided along with strain information including location and year of isolation. Phylogenetic tree was constructed using the neighbor-joining method with pairwise distance estimation following ClustalW alignment program. (B) Matrix panel shows the percent identity in the upper right <t>and</t> <t>nucleotide</t> divergence in the lower left of aligned Powassan viral genomic sequences (ClustalW program, DNASTAR Lasergene MegAlign 15). (C) Graph shows the RT-qPCR analysis of POWV (LB), POWV ( M11665 ), and DTV (SPO) genome replication in U118 cells 24 and 48 hpi. The y axis shows genome copies per 50 ng of total RNA in log scale. Error bars represent the SD of three biological replicates. Student’s t test. ∗ p < 0.01, ∗∗ p < 0.001, ∗∗∗ p < 0.0001, ∗∗∗∗ p < 0.00001. (D) Representative immunofluorescence images of mock and LB-, DTV- ( M11665 ), and DTV- (SPO) infected U118 neuronal cells detected by viral NS1 at 24 and 48 hpi (scale bars, 25 μm). Red, NS1 viral protein; blue, DAPI. (E) Western blot analysis of infected cell lysates probed with the anti-M protein antibody targeting LB, DTV ( M11665 ), and DTV (SPO) at 24 and 48 hpi. Cells were infected with each virus at an MOI of 1. Two independent experiments were conducted. Representative data are presented.
Lasergene Genomics Suite Version 15, supplied by DNASTAR, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lasergene+genomics+suite+version+15/pmc08500222-170-9-14?v=DNASTAR
Average 94 stars, based on 1 article reviews
lasergene genomics suite version 15 - by Bioz Stars, 2026-08
94/100 stars
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Genomic sequence analysis and infectious U118 neuronal cell culture system (A) Phylogenetic analysis of five Powassan viral genome sequences from known lineage I and II strains. GenBank accession numbers are provided along with strain information including location and year of isolation. Phylogenetic tree was constructed using the neighbor-joining method with pairwise distance estimation following ClustalW alignment program. (B) Matrix panel shows the percent identity in the upper right and nucleotide divergence in the lower left of aligned Powassan viral genomic sequences (ClustalW program, DNASTAR Lasergene MegAlign 15). (C) Graph shows the RT-qPCR analysis of POWV (LB), POWV ( M11665 ), and DTV (SPO) genome replication in U118 cells 24 and 48 hpi. The y axis shows genome copies per 50 ng of total RNA in log scale. Error bars represent the SD of three biological replicates. Student’s t test. ∗ p < 0.01, ∗∗ p < 0.001, ∗∗∗ p < 0.0001, ∗∗∗∗ p < 0.00001. (D) Representative immunofluorescence images of mock and LB-, DTV- ( M11665 ), and DTV- (SPO) infected U118 neuronal cells detected by viral NS1 at 24 and 48 hpi (scale bars, 25 μm). Red, NS1 viral protein; blue, DAPI. (E) Western blot analysis of infected cell lysates probed with the anti-M protein antibody targeting LB, DTV ( M11665 ), and DTV (SPO) at 24 and 48 hpi. Cells were infected with each virus at an MOI of 1. Two independent experiments were conducted. Representative data are presented.

Journal: iScience

Article Title: Phosphatidylserine receptors TIM-1 and AXL mediate tick-borne Powassan virus entry

doi: 10.1016/j.isci.2025.113930

Figure Lengend Snippet: Genomic sequence analysis and infectious U118 neuronal cell culture system (A) Phylogenetic analysis of five Powassan viral genome sequences from known lineage I and II strains. GenBank accession numbers are provided along with strain information including location and year of isolation. Phylogenetic tree was constructed using the neighbor-joining method with pairwise distance estimation following ClustalW alignment program. (B) Matrix panel shows the percent identity in the upper right and nucleotide divergence in the lower left of aligned Powassan viral genomic sequences (ClustalW program, DNASTAR Lasergene MegAlign 15). (C) Graph shows the RT-qPCR analysis of POWV (LB), POWV ( M11665 ), and DTV (SPO) genome replication in U118 cells 24 and 48 hpi. The y axis shows genome copies per 50 ng of total RNA in log scale. Error bars represent the SD of three biological replicates. Student’s t test. ∗ p < 0.01, ∗∗ p < 0.001, ∗∗∗ p < 0.0001, ∗∗∗∗ p < 0.00001. (D) Representative immunofluorescence images of mock and LB-, DTV- ( M11665 ), and DTV- (SPO) infected U118 neuronal cells detected by viral NS1 at 24 and 48 hpi (scale bars, 25 μm). Red, NS1 viral protein; blue, DAPI. (E) Western blot analysis of infected cell lysates probed with the anti-M protein antibody targeting LB, DTV ( M11665 ), and DTV (SPO) at 24 and 48 hpi. Cells were infected with each virus at an MOI of 1. Two independent experiments were conducted. Representative data are presented.

Article Snippet: Phylogenetic tree was constructed using the neighbor-joining method with pairwise distance estimation following ClustalW alignment program. (B) Matrix panel shows the percent identity in the upper right and nucleotide divergence in the lower left of aligned Powassan viral genomic sequences (ClustalW program, DNASTAR Lasergene MegAlign 15). (C) Graph shows the RT-qPCR analysis of POWV (LB), POWV ( M11665 ), and DTV (SPO) genome replication in U118 cells 24 and 48 hpi.

Techniques: Sequencing, Cell Culture, Isolation, Construct, Genomic Sequencing, Quantitative RT-PCR, Immunofluorescence, Infection, Western Blot, Virus